fmb microarray scanner calibration slides  (Full Moon BioSystems)

Journal: Nucleic Acids Research

Article Title: Beyond Affymetrix arrays: expanding the set of known hybridization isotherms and observing pre-wash signal intensities

doi: 10.1093/nar/gkp1122

Figure Lengend Snippet: Hybridization isotherms obtained on two different microarray platforms for rRNA target and Probe 596. Left and middle panels represent replicated Erie microarrays while the right panels represent VWR microarrays. Different rows represent distinct spotted probe concentrations. Each data point is the mean of at least six replicated spots. Error is measured as standard deviation. Isotherms from Erie microarrays are linear (but plotted on a log scale) while VWR microarrays followed the Langmuir model.

Article Snippet: The VersArray ChipReader 10 μm System scanner (Bio-Rad Laboratories, USA) was calibrated using a microarray scanner calibration slide from Full Moon Biosystems, and optimal PMT settings were chosen for scanning Erie and VWR microarrays.

Techniques: Hybridization, Microarray, Standard Deviation

Journal: Nucleic Acids Research

Article Title: Beyond Affymetrix arrays: expanding the set of known hybridization isotherms and observing pre-wash signal intensities

doi: 10.1093/nar/gkp1122

Figure Lengend Snippet: K versus spotted probe concentration for the pure rRNA target and the same probes ( n = 95) but different microarray platforms. Top and middle panels, replicated Erie microarrays; Bottom panel, VWR microarray. Red line shows the general trend based on the mean.

Article Snippet: The VersArray ChipReader 10 μm System scanner (Bio-Rad Laboratories, USA) was calibrated using a microarray scanner calibration slide from Full Moon Biosystems, and optimal PMT settings were chosen for scanning Erie and VWR microarrays.

Techniques: Concentration Assay, Microarray

Journal: Nucleic Acids Research

Article Title: Beyond Affymetrix arrays: expanding the set of known hybridization isotherms and observing pre-wash signal intensities

doi: 10.1093/nar/gkp1122

Figure Lengend Snippet: The effect of successive stringent washes on probe fluorescent densities recorded at equilibrium. Samples were hybridized for 4 h (Panels in rows A and B) or 24 h (Panels C and D in rows) with active mixing and were either pure rRNA (Panels in rows A and C) or rRNA mixed with unlabeled tRNA (Panels in rows B and D). These results are based on at least two independent microarray experiments. Note that the number of points is not the same for different washes because converting signal intensity to fluorescent density sometimes generated numbers that were nonphysical.

Article Snippet: The VersArray ChipReader 10 μm System scanner (Bio-Rad Laboratories, USA) was calibrated using a microarray scanner calibration slide from Full Moon Biosystems, and optimal PMT settings were chosen for scanning Erie and VWR microarrays.

Techniques: Microarray, Generated